UBE2L3 recombinant monoclonal antibody, clone R02-4B2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human UBE2L3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human UBE2L3.
Theoretical MW (kDa)
Calculated MW: 18 kD
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 3T3 whole cell lysate and Lane 2: mouse kidney tissue with UBE2L3 recombinant monoclonal antibody, clone R02-4B2 (Cat # RAB04422).Immunocytochemistry
Immunocytochemistry staining of HeLa cells with UBE2L3 recombinant monoclonal antibody, clone R02-4B2 (Cat # RAB04422) (green). DAPI was stained the cell nucleus (blue).Immunofluorescence
Immunoprecipitation
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Gene Info — UBE2L3
Entrez GeneID
7332Protein Accession#
P68036Gene Name
UBE2L3
Gene Alias
E2-F1, L-UBC, UBCH7, UbcM4
Gene Description
ubiquitin-conjugating enzyme E2L 3
Omim ID
603721Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
ubiquitin carrier protein|ubiquitin-conjugating enzyme UBCH7|ubiquitin-protein ligase
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Interactome
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Disease
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