CD8A recombinant monoclonal antibody, clone 2F2
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: MCF-7 whole cell lysate, Lane 2: THP-1 whole cell lysate, Lane 3: PC3 whole cell lysate, Lane 4: 293 whole cell lysate, Lane 5: HepG2 whole cell lysate and Lane 6: A549 whole cell lysate with CD8A recombinant monoclonal antibody, clone 2F2 (Cat # RAB04395).
Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with CD8A recombinant monoclonal antibody, clone 2F2 (Cat # RAB04395) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human breast cancer with CD8A recombinant monoclonal antibody, clone 2F2 (Cat # RAB04395) (diluated at 1:100).
Immunoprecipitation
Immunoprecipitation analysis of HepG2 cell lysate with CD8A recombinant monoclonal antibody, clone 2F2 (Cat # RAB04395).
Lane 1: rabbit control IgG, Lane 2: RAB04395 precipitates and Lane 3: Input (HepG2 whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD8A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD8A.
Theoretical MW (kDa)
Calculated MW: 26, 2
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: MCF-7 whole cell lysate, Lane 2: THP-1 whole cell lysate, Lane 3: PC3 whole cell lysate, Lane 4: 293 whole cell lysate, Lane 5: HepG2 whole cell lysate and Lane 6: A549 whole cell lysate with CD8A recombinant monoclonal antibody, clone 2F2 (Cat # RAB04395).Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with CD8A recombinant monoclonal antibody, clone 2F2 (Cat # RAB04395) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human breast cancer with CD8A recombinant monoclonal antibody, clone 2F2 (Cat # RAB04395) (diluated at 1:100).Immunoprecipitation
Immunoprecipitation analysis of HepG2 cell lysate with CD8A recombinant monoclonal antibody, clone 2F2 (Cat # RAB04395).
Lane 1: rabbit control IgG, Lane 2: RAB04395 precipitates and Lane 3: Input (HepG2 whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD8A
Entrez GeneID
925Protein Accession#
P01732Gene Name
CD8A
Gene Alias
CD8, Leu2, MAL, p32
Gene Description
CD8a molecule
Gene Ontology
HyperlinkGene Summary
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD8 antigen alpha polypeptide|CD8 antigen, alpha polypeptide (p32)|Leu2 T-lymphocyte antigen|OKT8 T-cell antigen|T cell co-receptor|T-cell antigen Leu2|T-cell surface glycoprotein CD8 alpha chain|T-lymphocyte differentiation antigen T8/Leu-2|T8 T-cell ant
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Interactome
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Pathway
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