BRD4 recombinant monoclonal antibody, clone 1E5
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: 293T whole cell lysate, Lane 3: HepG2 whole cell lysate and Lane 4: A549 whole cell lysate with BRD4 recombinant monoclonal antibody, clone 1E5 (Cat # RAB04386).
Immunohistochemistry
Immunohistochemical staining of human brain tissue with BRD4 recombinant monoclonal antibody, clone 1E5 (Cat # RAB04386) (diluated at 1:100).
Immunohistochemistry
Immunohistochemical staining of human breast carncer with BRD4 recombinant monoclonal antibody, clone 1E5 (Cat # RAB04386) (diluated at 1:100).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BRD4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human BRD4.
Theoretical MW (kDa)
Calculated MW: 153,
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: 293T whole cell lysate, Lane 3: HepG2 whole cell lysate and Lane 4: A549 whole cell lysate with BRD4 recombinant monoclonal antibody, clone 1E5 (Cat # RAB04386).Immunohistochemistry
Immunohistochemical staining of human brain tissue with BRD4 recombinant monoclonal antibody, clone 1E5 (Cat # RAB04386) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human breast carncer with BRD4 recombinant monoclonal antibody, clone 1E5 (Cat # RAB04386) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
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Gene Info — BRD4
Entrez GeneID
23476Protein Accession#
O60885Gene Name
BRD4
Gene Alias
CAP, HUNK1, HUNKI, MCAP
Gene Description
bromodomain containing 4
Omim ID
608749Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
bromodomain-containing 4|bromodomain-containing protein 4|chromosome-associated protein
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Interactome
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