BRD4 recombinant monoclonal antibody, clone RAB-C131
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More Files
- More Functions
Western Blot
Western blot analysis of K562 cell lysate with BRD4 recombinant monoclonal antibody, clone RAB-C131 (Cat # RAB03104).
Immunofluorescence
Immunofluorescent staining of HeLa cells with BRD4 recombinant monoclonal antibody, clone RAB-C131 (Cat # RAB03104).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03104 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom RAB03104- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of HepG2 cells with BRD4 recombinant monoclonal antibody, clone RAB-C131 (Cat # RAB03104).
HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03104 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BRD4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant human BRD4 protein under non-denaturing conditions.
Reactivity
Human
Form
Liquid
Isotype
IgG kappa
Recommend Usage
ChIP
ELISA
Flow Cytometry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
ChIP
Western Blot
Western blot analysis of K562 cell lysate with BRD4 recombinant monoclonal antibody, clone RAB-C131 (Cat # RAB03104).Immunofluorescence
Immunofluorescent staining of HeLa cells with BRD4 recombinant monoclonal antibody, clone RAB-C131 (Cat # RAB03104).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03104 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom RAB03104- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cells with BRD4 recombinant monoclonal antibody, clone RAB-C131 (Cat # RAB03104).
HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03104 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — BRD4
Entrez GeneID
23476Gene Name
BRD4
Gene Alias
CAP, HUNK1, HUNKI, MCAP
Gene Description
bromodomain containing 4
Omim ID
608749Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
bromodomain-containing 4|bromodomain-containing protein 4|chromosome-associated protein
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Interactome
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