HMGB1 recombinant monoclonal antibody, clone 1A1
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: Jurkat whole cell lysate, Lane 4: 293T whole cell lysate, Lane 5: MCF-7 whole cell lysate and Lane 6: HL-60 whole cell lysate with HMGB1 recombinant monoclonal antibody, clone 1A1 (Cat # RAB04382).
Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with HMGB1 recombinant monoclonal antibody, clone 1A1 (Cat # RAB04382) (diluated at 1:100).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HMGB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HMGB1.
Theoretical MW (kDa)
Calculated MW: 25 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: Jurkat whole cell lysate, Lane 4: 293T whole cell lysate, Lane 5: MCF-7 whole cell lysate and Lane 6: HL-60 whole cell lysate with HMGB1 recombinant monoclonal antibody, clone 1A1 (Cat # RAB04382).Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with HMGB1 recombinant monoclonal antibody, clone 1A1 (Cat # RAB04382) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HMGB1
Entrez GeneID
3146Protein Accession#
P09429Gene Name
HMGB1
Gene Alias
DKFZp686A04236, HMG1, HMG3, SBP-1
Gene Description
high-mobility group box 1
Omim ID
163905Gene Ontology
HyperlinkOther Designations
Amphoterin|OTTHUMP00000018199|OTTHUMP00000190860|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group protein 1|high-mobility group (nonhistone chromosomal) protein 1
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Interactome
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Pathway
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Disease
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