HMGB1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates with HMGB1 recombinant monoclonal antibody (Cat # RAB02425).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using HMGB1 recombinant monoclonal antibody (Cat # RAB02425). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.111). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against recombinant HMGB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human HMGB1.
Theoretical MW (kDa)
26
Reactivity
Human, Mouse, Rat
Specificity
Recognizes endogenous levels of HMGB1 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates with HMGB1 recombinant monoclonal antibody (Cat # RAB02425).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using HMGB1 recombinant monoclonal antibody (Cat # RAB02425). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.111). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Gene Info — HMGB1
Entrez GeneID
3146Protein Accession#
P09429Gene Name
HMGB1
Gene Alias
DKFZp686A04236, HMG1, HMG3, SBP-1
Gene Description
high-mobility group box 1
Omim ID
163905Gene Ontology
HyperlinkOther Designations
Amphoterin|OTTHUMP00000018199|OTTHUMP00000190860|Sulfoglucuronyl carbohydrate binding protein|high mobility group box 1|high mobility group protein 1|high-mobility group (nonhistone chromosomal) protein 1
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Interactome
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Pathway
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Disease
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