CDC5L recombinant monoclonal antibody, clone 1E3
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: 293 whole cell lysate, Lane 5: K562 whole cell lysate and Lane 6: Jurkat whole cell lysate with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314).
Immunohistochemistry
Immunohistochemical staining of human pancreatic tissue with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314) (diluated at 1:126.5).
Immunohistochemistry
Immunohistochemical staining of human appendix tissue with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314) (diluated at 1:126.5)
Immunofluorescence
Immunofluorescent staining of A549 cells with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314) (diluated at 1:42). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314).
Lane 1: rabbit control IgG, Lane 2: RAB04314 precipitates and Lane 3: Input (Hela whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDC5L.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CDC5L.
Theoretical MW (kDa)
Calculated MW: 93 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: 293 whole cell lysate, Lane 5: K562 whole cell lysate and Lane 6: Jurkat whole cell lysate with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314).Immunohistochemistry
Immunohistochemical staining of human pancreatic tissue with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314) (diluated at 1:126.5).Immunohistochemistry
Immunohistochemical staining of human appendix tissue with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314) (diluated at 1:126.5)Immunofluorescence
Immunofluorescent staining of A549 cells with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314) (diluated at 1:42). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with CDC5L recombinant monoclonal antibody, clone 1E3 (Cat # RAB04314).
Lane 1: rabbit control IgG, Lane 2: RAB04314 precipitates and Lane 3: Input (Hela whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC5L
Entrez GeneID
988Protein Accession#
Q99459Gene Name
CDC5L
Gene Alias
CEF1, KIAA0432, PCDC5RP, dJ319D22.1, hCDC5
Gene Description
CDC5 cell division cycle 5-like (S. pombe)
Omim ID
602868Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq
Other Designations
CDC5 (cell division cycle 5, S. pombe, homolog)-like|CDC5-like|Cdc5-related protein|Cell division cycle 5, S. pombe, homolog-like|OTTHUMP00000016529|dJ319D22.1 (CDC5-like protein)
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Interactome
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Disease
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