FTO recombinant monoclonal antibody, clone 4G9
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:293T whole cell lysate, Lane 2: SH-SY5Y whole cell lysate, Lane 3: Raji whole cell lysate, Lane 4: Cola320 whole cell lysate, Lane 5: A549 whole cell lysate and Lane 6: PC3 whole cell lysate with FTO recombinant monoclonal antibody, clone 4G9 (Cat # RAB04311).
Immunohistochemistry
Immunohistochemical staining of human glioma cancer with FTO recombinant monoclonal antibody, clone 4G9 (Cat # RAB04311) (diluated at 1:70).
Immunohistochemistry
Immunohistochemical staining of human skeletal muscle tissue with FTO recombinant monoclonal antibody, clone 4G9 (Cat # RAB04311) (diluated at 1:70).
Immunofluorescence
Immunofluorescent staining of Hela cells with FTO recombinant monoclonal antibody, clone 4G9 (Cat # RAB04311) (diluated at 1:23). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FTO.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human FTO.
Theoretical MW (kDa)
Calculated MW: 59, 1
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:293T whole cell lysate, Lane 2: SH-SY5Y whole cell lysate, Lane 3: Raji whole cell lysate, Lane 4: Cola320 whole cell lysate, Lane 5: A549 whole cell lysate and Lane 6: PC3 whole cell lysate with FTO recombinant monoclonal antibody, clone 4G9 (Cat # RAB04311).Immunohistochemistry
Immunohistochemical staining of human glioma cancer with FTO recombinant monoclonal antibody, clone 4G9 (Cat # RAB04311) (diluated at 1:70).Immunohistochemistry
Immunohistochemical staining of human skeletal muscle tissue with FTO recombinant monoclonal antibody, clone 4G9 (Cat # RAB04311) (diluated at 1:70).Immunofluorescence
Immunofluorescent staining of Hela cells with FTO recombinant monoclonal antibody, clone 4G9 (Cat # RAB04311) (diluated at 1:23). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — FTO
Entrez GeneID
79068Protein Accession#
Q9C0B1Gene Name
FTO
Gene Alias
KIAA1752, MGC5149
Gene Description
fat mass and obesity associated
Gene Ontology
HyperlinkGene Summary
The exact function of this gene is not know. Studies in mice suggest that it may be involved in nucleic acid demethylation, and that its mRNA level is regulated by feeding and fasting. Genomewide association studies of type 2 diabetes indicate this gene as a diabetes susceptibility locus. Mutation in this gene has been associated with growth retardation, developmental delay, coarse facies, and early death. [provided by RefSeq
Other Designations
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Interactome
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Disease
- Anorexia Nervosa
- Appetite
- Asthma
- Atherosclerosis
- Birth Weight
- Calcinosis
- Carcinoma
- Cardiovascular Diseases
- Cataract
+ View More Disease
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