FTO recombinant monoclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FTO.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human FTO.
Theoretical MW (kDa)
60
Reactivity
Human
Specificity
Recognizes endogenous levels of FTO protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of Hela (A), Jurkat (B) whole cell lysates with FTO recombinant monoclonal antibody (Cat # RAB02500).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using FTO recombinant monoclonal antibody (Cat # RAB02500). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.82). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with FTO recombinant monoclonal antibody (Cat # RAB02500). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — FTO
Entrez GeneID
79068Protein Accession#
Q9C0B1Gene Name
FTO
Gene Alias
KIAA1752, MGC5149
Gene Description
fat mass and obesity associated
Gene Ontology
HyperlinkGene Summary
The exact function of this gene is not know. Studies in mice suggest that it may be involved in nucleic acid demethylation, and that its mRNA level is regulated by feeding and fasting. Genomewide association studies of type 2 diabetes indicate this gene as a diabetes susceptibility locus. Mutation in this gene has been associated with growth retardation, developmental delay, coarse facies, and early death. [provided by RefSeq
Other Designations
-
-
Interactome
-
Disease
- Anorexia Nervosa
- Appetite
- Asthma
- Atherosclerosis
- Birth Weight
- Calcinosis
- Carcinoma
- Cardiovascular Diseases
- Cataract
+ View More Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com