PRKAA2 (phospho Thr172) recombinant monoclonal antibody, clone 4F4
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More Files
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Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 50 nM/60 mins), Lane 2: 293 whole cell lysate (not treated), Lane 3: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 4: A549 whole cell lysate (not treated) with PRKAA2 (phospho Thr172) recombinant monoclonal antibody, clone 4F4 (Cat # RAB04245).
Immunofluorescence
Immunofluorescent staining of A549 cells with PRKAA2 (phospho Thr172) recombinant monoclonal antibody, clone 4F4 (Cat # RAB04245) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PRKAA2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr172 of human PRKAA2.
Theoretical MW (kDa)
Calculated MW: 62 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 50 nM/60 mins), Lane 2: 293 whole cell lysate (not treated), Lane 3: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 4: A549 whole cell lysate (not treated) with PRKAA2 (phospho Thr172) recombinant monoclonal antibody, clone 4F4 (Cat # RAB04245).Immunofluorescence
Immunofluorescent staining of A549 cells with PRKAA2 (phospho Thr172) recombinant monoclonal antibody, clone 4F4 (Cat # RAB04245) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKAA2
Entrez GeneID
5563Protein Accession#
P54646Gene Name
PRKAA2
Gene Alias
AMPK, AMPK2, PRKAA
Gene Description
protein kinase, AMP-activated, alpha 2 catalytic subunit
Omim ID
600497Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-2 chain|AMP-activated protein kinase alpha 2 catalytic subunit|AMPK-alpha-2 chain|OTTHUMP00000009993
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