EIF4E (phospho S209) recombinant monoclonal antibody, clone 1F3
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More Files
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Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 100 nM/60 mins), Lane 2: 293 whole cell lysate (not treated), Lane 3: HepG2 whole cell lysate (treated with Calyculin A 100 nM/60 mins), Lane 4: HepG2 whole cell lysate (not treated) with EIF4E (phospho S209) recombinant monoclonal antibody, clone 1F3 (Cat # RAB04223).
Immunohistochemistry
Immunohistochemical staining of human breast cancer with EIF4E (phospho S209) recombinant monoclonal antibody, clone 1F3 (Cat # RAB04223) (diluated at 1:100).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EIF4E.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S209 of human EIF4E.
Theoretical MW (kDa)
Calculated MW: 25 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293 whole cell lysate (treated with Calyculin A 100 nM/60 mins), Lane 2: 293 whole cell lysate (not treated), Lane 3: HepG2 whole cell lysate (treated with Calyculin A 100 nM/60 mins), Lane 4: HepG2 whole cell lysate (not treated) with EIF4E (phospho S209) recombinant monoclonal antibody, clone 1F3 (Cat # RAB04223).Immunohistochemistry
Immunohistochemical staining of human breast cancer with EIF4E (phospho S209) recombinant monoclonal antibody, clone 1F3 (Cat # RAB04223) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EIF4E
Entrez GeneID
1977Protein Accession#
P06730Gene Name
EIF4E
Gene Alias
CBP, EIF4E1, EIF4EL1, EIF4F, MGC111573
Gene Description
eukaryotic translation initiation factor 4E
Omim ID
133440Gene Ontology
HyperlinkGene Summary
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
Other Designations
eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
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Interactome
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Disease
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