EIF4E (phospho S209) monoclonal antibody, clone DBD-5
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of (1) HEK293 cell lysate, (2) Mouse spleen lysate using EIF4E (phospho S209) monoclonal antibody, clone DBD-5.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using EIF4E (phospho S209) monoclonal antibody, clone DBD-5.
-
Specification
Product Description
Rabbit monoclonal antibody raised against synthetic phosphopeptide of human EIF4E.
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding S209 f human EIF4E.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.4-0.5 mg/mL BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western Blot analysis of (1) HEK293 cell lysate, (2) Mouse spleen lysate using EIF4E (phospho S209) monoclonal antibody, clone DBD-5.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using EIF4E (phospho S209) monoclonal antibody, clone DBD-5.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
-
Gene Info — EIF4E
Entrez GeneID
1977Protein Accession#
P06730Gene Name
EIF4E
Gene Alias
CBP, EIF4E1, EIF4EL1, EIF4F, MGC111573
Gene Description
eukaryotic translation initiation factor 4E
Omim ID
133440Gene Ontology
HyperlinkGene Summary
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM
Other Designations
eIF-4F 25 kDa subunit|eukaryotic translation initiation factor 4E-like 1|mRNA cap-binding protein
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com