MYB (phospho S11) recombinant monoclonal antibody, clone 3F4
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More Files
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Immunofluorescence
Immunofluorescent staining of Hela cells with MYB (phospho S11) recombinant monoclonal antibody, clone 3F4 (Cat # RAB04222) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MYB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S11 of human MYB.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescent staining of Hela cells with MYB (phospho S11) recombinant monoclonal antibody, clone 3F4 (Cat # RAB04222) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — MYB
Entrez GeneID
4602Protein Accession#
P10242Gene Name
MYB
Gene Alias
Cmyb, c-myb, c-myb_CDS, efg
Gene Description
v-myb myeloblastosis viral oncogene homolog (avian)
Omim ID
189990Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
Avian myeloblastosis viral (v-myb) oncogene homolog|OTTHUMP00000017258|c-myb protein (140 AA)|c-myb10A_CDS|c-myb13A_CDS|c-myb14A_CDS|c-myb8B_CDS|v-myb avian myeloblastosis viral oncogene homolog|v-myb myeloblastosis viral oncogene homolog
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Interactome
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Disease
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