CD99 recombinant monoclonal antibody, clone 9A5
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More Files
- More Functions
Western Blot
Western blot analysis of Jurkat whole cell lysate with CD99 recombinant monoclonal antibody, clone 9A5 (Cat # RAB04214).
Immunohistochemistry
Immunohistochemical staining of human pancreatic tissue with CD99 recombinant monoclonal antibody, clone 9A5 (Cat # RAB04214) (diluated at 1:100).
Flow Cytometry
Flow cytometric analysis of Jurkat cells with CD99 recombinant monoclonal antibody, clone 9A5 (Cat # RAB04214) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD99.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD99.
Theoretical MW (kDa)
Calculated MW: 28 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Jurkat whole cell lysate with CD99 recombinant monoclonal antibody, clone 9A5 (Cat # RAB04214).Immunohistochemistry
Immunohistochemical staining of human pancreatic tissue with CD99 recombinant monoclonal antibody, clone 9A5 (Cat # RAB04214) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells with CD99 recombinant monoclonal antibody, clone 9A5 (Cat # RAB04214) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — CD99
Entrez GeneID
4267Protein Accession#
P14209Gene Name
CD99
Gene Alias
MIC2, MIC2X, MIC2Y
Gene Description
CD99 molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD99 antigen|E2 antigen|MIC2 (monoclonal antibody 12E7)|OTTHUMP00000022840|T-cell surface glycoprotein E2|antigen identified by monoclonal 12E7, Y homolog|antigen identified by monoclonal antibodies 12E7, F21 and O13|surface antigen MIC2
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Interactome
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Pathway
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Disease
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