CD99 recombinant monoclonal antibody, clone MIC2/1495R
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD99 protein.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human CD99 protein
Reactivity
Human
Form
Liquid
Conjugation
Unconjugated
Purification
Protein A affinity chromatography
Concentration
0.2 mg/mL
Isotype
IgG
Recommend Usage
Flow cytometry (2-4 ug/million cells in 0.1mL)
Immunofluorescence (2-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(5-10 ug/mL for 30 min at RT)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage Instruction
Store at 2~8°C.
Aliquot to avoid repeated freezing and thawing.Note
Optimal dilutions for each application to be determined by the researcher
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of human Ewing's sarcoma with anti-CD99 recombinant monoclonal antibody, clone MIC2/1495R (Cat # RAB03807).Immunofluorescence
Flow Cytometry
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Gene Info — CD99
Entrez GeneID
4267Protein Accession#
P14209Gene Name
CD99
Gene Alias
MIC2, MIC2X, MIC2Y
Gene Description
CD99 molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD99 antigen|E2 antigen|MIC2 (monoclonal antibody 12E7)|OTTHUMP00000022840|T-cell surface glycoprotein E2|antigen identified by monoclonal 12E7, Y homolog|antigen identified by monoclonal antibodies 12E7, F21 and O13|surface antigen MIC2
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