Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11
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More Files
- More Functions
Western Blot
Western blot analysis of Hela whole cell lysate with Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11 (Cat # RAB04207).
Immunocytochemistry
Immunocytochemical staining of HeLa cells with Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11 (Cat # RAB04207) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11 (Cat # RAB04207) (diluated at 1:84). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cells with Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11 (Cat # RAB04207) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human Histone H2B type 1-B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic acetyl peptide corresponding to residues surrounding K20 of human histone H2B type 1-B.
Theoretical MW (kDa)
Calculated MW: 15 kD
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry (1:50-1:500)
Immunofluorescence (1:30-1:200)
Western Blot (1:5000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Hela whole cell lysate with Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11 (Cat # RAB04207).Immunocytochemistry
Immunocytochemical staining of HeLa cells with Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11 (Cat # RAB04207) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11 (Cat # RAB04207) (diluated at 1:84). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cells with Histone H2B type 1-B (acetyl K20) recombinant monoclonal antibody, clone 21F11 (Cat # RAB04207) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — HIST1H2BB
Entrez GeneID
3018Protein Accession#
P33778Gene Name
HIST1H2BB
Gene Alias
H2B.1, H2B/f, H2BFF, MGC119804
Gene Description
histone cluster 1, H2bb
Omim ID
602803Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H2B histone family, member F|OTTHUMP00000016138|histone 1, H2bb
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Interactome
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Pathway
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