LOXL2 recombinant monoclonal antibody, clone 2E5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate; Lane 2: PC3 whole cell lysate; Lane 3: Mouse brain tissue; Lane 4: Rat brain tissue.
Immunohistochemistry
Immunohistochemistry image of LOXL2 recombinant monoclonal antibody, clone 2E5 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of HepG2 Cells with LOXL2 recombinant monoclonal antibody, clone 2E5 at 1:50, counter-stained with DAPI.
Flow Cytometry
Overlay histogram showing A549 cells stained with LOXL2 recombinant monoclonal antibody, clone 2E5 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human, mouse and rat LOXL2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human LOXL2.
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate; Lane 2: PC3 whole cell lysate; Lane 3: Mouse brain tissue; Lane 4: Rat brain tissue.Immunohistochemistry
Immunohistochemistry image of LOXL2 recombinant monoclonal antibody, clone 2E5 diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of HepG2 Cells with LOXL2 recombinant monoclonal antibody, clone 2E5 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing A549 cells stained with LOXL2 recombinant monoclonal antibody, clone 2E5 (red line) at 1:50. -
Gene Info — LOXL2
Entrez GeneID
4017Protein Accession#
Q9Y4K0Gene Name
LOXL2
Gene Alias
LOR2, WS9-14
Gene Description
lysyl oxidase-like 2
Omim ID
606663Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq
Other Designations
lysyl oxidase homolog 2|lysyl oxidase related 2
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Interactome
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Disease
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