DNM2 recombinant monoclonal antibody, clone 1D8
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: HL60 whole cell lysate; Lane 2: Rat brain tissue.
Immunofluorescence
Immunofluorescence staining of Hela Cells with DNM2 recombinant monoclonal antibody, clone 1D8 at 1:50, counter-stained with DAPI.
Flow Cytometry
Overlay histogram showing Hela cells stained with DNM2 recombinant monoclonal antibody, clone 1D8 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and rat DNM2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human DNM2.
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HL60 whole cell lysate; Lane 2: Rat brain tissue.Immunofluorescence
Immunofluorescence staining of Hela Cells with DNM2 recombinant monoclonal antibody, clone 1D8 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing Hela cells stained with DNM2 recombinant monoclonal antibody, clone 1D8 (red line) at 1:50. -
Gene Info — DNM2
Entrez GeneID
1785Protein Accession#
P50570Gene Name
DNM2
Gene Alias
CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII
Gene Description
dynamin 2
Gene Ontology
HyperlinkGene Summary
Dynamins represent one of the subfamilies of GTP-binding proteins. These proteins share considerable sequence similarity over the N-terminal portion of the molecule, which contains the GTPase domain. Dynamins are associated with microtubules. They have been implicated in cell processes such as endocytosis and cell motility, and in alterations of the membrane that accompany certain activities such as bone resorption by osteoclasts. Dynamins bind many proteins that bind actin and other cytoskeletal proteins. Dynamins can also self-assemble, a process that stimulates GTPase activity. Four alternatively spliced transcripts encoding different proteins have been described. Additional alternatively spliced transcripts may exist, but their full-length nature has not been determined. [provided by RefSeq
Other Designations
dynamin II
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Interactome
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Pathway
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Disease
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