RHD recombinant monoclonal antibody, clone D7C2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against a Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to a Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line
Reactivity
Human
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG
Recommend Usage
ADCC
Flow cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
For research use only.
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Applications
Immunofluorescence
Immunofluorescence analysis of RHD (green) in K562 cells using human anti-Rhesus D recombinant monoclonal antibody, clone D7C2 (Cat # RAB03837), and DAPI(blue).Flow Cytometry
Flow-cytometry analysis of RHD in K562 cells using human anti-Rhesus D recombinant monoclonal antibody, clone D7C2 (Cat # RAB03837). -
Gene Info — RHD
Entrez GeneID
6007Protein Accession#
Q02161?Gene Name
RHD
Gene Alias
CD240D, DIIIc, MGC165007, RH, RH30, RHCED, RHDVA(TT), RHDel, RHPII, RHXIII, Rh4, RhDCw, RhII, RhK562-II, RhPI
Gene Description
Rh blood group, D antigen
Omim ID
111680Gene Ontology
HyperlinkGene Summary
The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
D antigen (DCS)|OTTHUMP00000003536|RH polypeptide 2|Rh blood group D antigen|Rh blood group antigen Evans|Rhesus system D polypeptide
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Interactome
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Disease
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