TUBB3 recombinant monoclonal antibody, clone S11B
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HeLa, Lane 2: A431, Lane3: HEK293 and Lane 4: MCF-7 cell lysates with TUBB3 recombinant monoclonal antibody, clone S11B (Cat # RAB03098).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Paraffin-embedded sections) of human cerebral cortex tissue with TUBB3 recombinant monoclonal antibody, clone S11B (Cat # RAB03098) at 4 ug/mL.
Immunofluorescence
Immunofluoresent staining of HeLa cells with TUBB3 recombinant monoclonal antibody, clone S11B (Cat # RAB03098).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of RAB03098 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03098- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Hela cells with the TUBB3 recombinant monoclonal antibody, clone S11B (Cat # RAB03098).
HeLa cells were stained with unimmunized r antibody (black line) or the rmeric version of RAB03098 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TUBB3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human MBP (microtubule-binding protein).
Reactivity
Human
Form
Liquid
Isotype
IgG lambda
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Lane 1: HeLa, Lane 2: A431, Lane3: HEK293 and Lane 4: MCF-7 cell lysates with TUBB3 recombinant monoclonal antibody, clone S11B (Cat # RAB03098).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Paraffin-embedded sections) of human cerebral cortex tissue with TUBB3 recombinant monoclonal antibody, clone S11B (Cat # RAB03098) at 4 ug/mL.Immunofluorescence
Immunofluoresent staining of HeLa cells with TUBB3 recombinant monoclonal antibody, clone S11B (Cat # RAB03098).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of RAB03098 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03098- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Hela cells with the TUBB3 recombinant monoclonal antibody, clone S11B (Cat # RAB03098).
HeLa cells were stained with unimmunized r antibody (black line) or the rmeric version of RAB03098 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer. -
Gene Info — TUBB3
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Interactome
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Pathway
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