AFP recombinant monoclonal antibody, clone AF5
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More Files
- More Functions
Western Blot
Western blot analysis of HepG2 cells lysates with AFP recombinant monoclonal antibody, clone AF5 (Cat # RAB03058).
Immunofluorescence
Immunofluoresent staining of HepG2 cells with AFP recombinant monoclonal antibody, clone AF5 (Cat # RAB03058).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells- permewith 0.15% Triton stained with the chimeric r version of RAB03058 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of HepG2 cells with AFP recombinant monoclonal antibody, clone AF5 (Cat # RAB03058).
HepG2 cells were stained with unimmunized r antibody (black line) or the rmeric version of RAB03058 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human AFP.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human amniotic fluid containing AFP.
Reactivity
Human
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of HepG2 cells lysates with AFP recombinant monoclonal antibody, clone AF5 (Cat # RAB03058).Immunofluorescence
Immunofluoresent staining of HepG2 cells with AFP recombinant monoclonal antibody, clone AF5 (Cat # RAB03058).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells- permewith 0.15% Triton stained with the chimeric r version of RAB03058 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.Flow Cytometry
Flow cytometric analysis of HepG2 cells with AFP recombinant monoclonal antibody, clone AF5 (Cat # RAB03058).
HepG2 cells were stained with unimmunized r antibody (black line) or the rmeric version of RAB03058 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer. -
Gene Info — AFP
Entrez GeneID
174Gene Name
AFP
Gene Alias
FETA, HPAFP
Gene Description
alpha-fetoprotein
Omim ID
104150Gene Ontology
HyperlinkGene Summary
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
Other Designations
OTTHUMP00000160480|alpha-1-fetoprotein|alpha-fetoglobulin
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Interactome
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Disease
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