Il2rb recombinant monoclonal antibody, clone 5H4
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More Files
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Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Il2rb recombinant monoclonal antibody, clone 5H4 (Cat # RAB03580).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03580 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03580
(B) DAPI
(C) Merged channels
(D) Isotype control
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Il2rb.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against rat myeloma cell line YB2/0, expressing truncated murine Il2rb.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Il2rb recombinant monoclonal antibody, clone 5H4 (Cat # RAB03580).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03580 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03580
(B) DAPI
(C) Merged channels
(D) Isotype controlFlow Cytometry
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Gene Info — Il2rb
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