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Western Blot

Western Blot analysis of human breast cancer tissue with ERBB2 recombinant monoclonal antibody, clone SER4 (Cat # RAB03523). Western Blot analysis the tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric mouse IgG version of RAB03523 at 2 ug/mL before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence

Immunofluorescenct staining of MCF-7 cells with ERBB2 recombinant monoclonal antibody, clone SER4 (Cat # RAB03523).
Immunofluorescence analysis of paraformaldehyde fixed MCF-7 cells stained with the chimeric mouse IgG version of RAB03523 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03523
(B) DAPI
(C) Merged channels
(D) Isotype control

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of MCF-7 cells with ERBB2 recombinant monoclonal antibody, clone SER4 (Cat # RAB03523).
The fixed MCF-7 cells were stained with anti-unknown specificity antibody (isotype control-black line) or the mouse IgG1 version of RAB03523 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Entrez GeneID

2064

Gene Name

ERBB2

Gene Alias

CD340, HER-2, HER-2/neu, HER2, NEU, NGL, TKR1

Gene Description

v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

Omim ID

137215 137800 164870 211980

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq

Other Designations

c-erb B2/neu protein|erbB-2|herstatin|neuroblastoma/glioblastoma derived oncogene homolog|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)