ERBB2 recombinant monoclonal antibody, clone FWP51
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human ERBB2.
Antibody Species
Mouse
Immunogen
Original antibody is raised against SKBR3 human breast tumour cells.
Reactivity
Human
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry
Fusion
Immunofluorescence
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of MCF-7 cells with ERBB2 recombinant monoclonal antibody, clone FWP51 (Cat # RAB03367).
Immunofluorescence analysis of paraformaldehyde fixed MCF-7 cells stained with the chimeric r version of RAB03367 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-unknown antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03367
(B) DAPI
(C) Merged channels
(D) Isotype controlImmunoprecipitation
Flow Cytometry
Flow cytometric analysis of MCF-7 cells with ERBB2 recombinant monoclonal antibody, clone FWP51 (Cat # RAB03367).
Paraformaldehyde fixed MCF-7 cells were stained with anti-unknown specificity antibody (isotype control-black line) or the mouse IgG1 version of RAB03367 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — ERBB2
Entrez GeneID
2064Gene Name
ERBB2
Gene Alias
CD340, HER-2, HER-2/neu, HER2, NEU, NGL, TKR1
Gene Description
v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq
Other Designations
c-erb B2/neu protein|erbB-2|herstatin|neuroblastoma/glioblastoma derived oncogene homolog|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
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Interactome
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