Tnfrsf18 recombinant monoclonal antibody, clone YGITR 860.103.5
Size:200 ug
Price:
USD $
850.00
Stock:
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Tnfrsf18.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse Tnfrsf18.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescenct staining of mouse splenocytes with Tnfrsf18 recombinant monoclonal antibody, clone YGITR 860.103.5 (Cat # RAB03488).
Immunofluorescence analysis of paraformaldehyde mouse splenocytes fixed on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric r version of RAB03488 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/mL), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.Flow Cytometry
Flow cytometric analysis of mouse spleenocyts with Tnfrsf18 recombinant monoclonal antibody, clone YGITR 860.103.5 (Cat # RAB03488).
Mouse spleenocyts were stained with an isotype control (Orange) or the chimeric r version version of RAB03488 at a concentration of 1 ug/mL for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing the bound antibody was detected using a AF488 conjugated donkey anti-ribody antibody and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Tnfrsf18
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