FSCN1 recombinant monoclonal antibody, clone SAIC-32C-205
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of Hela cells with FSCN1 recombinant monoclonal antibody, clone SAIC-32C-205 (Cat # RAB03476).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells permeabilized with 0.15% Triton stained with the chimeric r version of RAB03476 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03476
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of Hela cells with FSCN1 recombinant monoclonal antibody, clone SAIC-32C-205 (Cat # RAB03476).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03476 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FSCN1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against peptide "TVEEAENIAVTSGVVR" derived from Fascin conjugated to KLH.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
Immuno-MRM (multiple reaction monitoring)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of Hela cells with FSCN1 recombinant monoclonal antibody, clone SAIC-32C-205 (Cat # RAB03476).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells permeabilized with 0.15% Triton stained with the chimeric r version of RAB03476 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03476
(B) DAPI
(C) Merged channels
(D) Isotype controlFlow Cytometry
Flow cytometric analysis of Hela cells with FSCN1 recombinant monoclonal antibody, clone SAIC-32C-205 (Cat # RAB03476).
Paraformaldehyde fixed Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03476 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Immuno-MRM (multiple reaction monitoring)
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Gene Info — FSCN1
Entrez GeneID
6624Gene Name
FSCN1
Gene Alias
FLJ38511, SNL, p55
Gene Description
fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
Omim ID
602689Gene Ontology
HyperlinkGene Summary
O
Other Designations
Singed, drosophila, homolog-like|actin bundling protein|fascin 1|singed-like (fascin homolog, sea urchin)
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Interactome
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Disease
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