Cd86 recombinant monoclonal antibody, clone PO.3
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd86.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
Reactivity
Mouse
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG, kappa
Recommend Usage
Blocking
Flow Cytometry
Functional Study
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of Raw 264.7 cells with Cd86 recombinant monoclonal antibody, clone PO.3 (Cat # RAB03348).
Immunofluorescence analysis of Paraformaldehyde Raw 264.7 cells fixed on Shi-fix™ coverslips stained with the chimeric r version of RAB03348 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor®488 secondary antibody.
(A) RAB03348
(B) DAPI
(C) Merged channels
(D) Isotype controlFunctional Study
Blocking
Flow Cytometry
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Gene Info — Cd86
Entrez GeneID
12524Gene Name
Cd86
Gene Alias
B7, B7-2, B7.2, B70, CLS1, Cd28l2, ETC-1, Ly-58, Ly58, MB7, MB7-2, TS/A-2
Gene Description
CD86 antigen
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000015940|T-lymphocyte activation antigen CD86|activation B7-2 antigen|early T cell costimulatory molecule-1
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