CD74 recombinant monoclonal antibody, clone LN-2
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More Files
- More Functions
Western Blot
Western blot anaysis of Lane 1: Daudi cell lysate, Lane 2: human spleen lysate and Lane 3: human tonsil tissue lysate with CD74 recombinant monoclonal antibody, clone LN-2 (Cat # RAB03332).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescent staining of Daudi cells with CD74 recombinant monoclonal antibody, clone LN-2 (Cat # RAB03332).
Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03332 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03332- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Daudi cells with CD74 recombinant monoclonal antibody, clone LN-2 (Cat # RAB03332).
Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03332 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD74.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against SU-DHL-4 lymphoma cells.
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG kappa
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot anaysis of Lane 1: Daudi cell lysate, Lane 2: human spleen lysate and Lane 3: human tonsil tissue lysate with CD74 recombinant monoclonal antibody, clone LN-2 (Cat # RAB03332).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescent staining of Daudi cells with CD74 recombinant monoclonal antibody, clone LN-2 (Cat # RAB03332).
Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03332 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03332- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Daudi cells with CD74 recombinant monoclonal antibody, clone LN-2 (Cat # RAB03332).
Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03332 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — CD74
Entrez GeneID
972Gene Name
CD74
Gene Alias
DHLAG, HLADG, Ia-GAMMA
Gene Description
CD74 molecule, major histocompatibility complex, class II invariant chain
Omim ID
142790Gene Ontology
HyperlinkGene Summary
major histocompatibility complex
Other Designations
CD74 antigen|CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)|HLA-DR-gamma|Ia-associated invariant chain|MHC HLA-DR gamma chain|gamma chain of class II antigens
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Interactome
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Pathway
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Disease
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