Sell recombinant monoclonal antibody, clone MEL-14
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More Files
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Flow Cytometry
Flow cytometric analysis of mouse lymphocytes with Sell recombinant monoclonal antibody, clone MEL-14 (Cat # RAB03316).
Mouse lymphocytes were stained with an anti-CD44 control (3.0- panel A) or the rmeric version of RAB03316 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.
Flow Cytometry
Flow cytometric analysis of mouse splenocytes with Sell recombinant monoclonal antibody, clone MEL-14 (Cat # RAB03316).
Mouse splenocytes were stained with unimmunized r antibody (black line) or the rmeric version of RAB03316 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Sell.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against C3H/eb mouse B lymphoma 38C-13.
Reactivity
Mouse
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Blocking
Flow Cytometry
Immunohistochemistry
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry
Immunoprecipitation
Blocking
Flow Cytometry
Flow cytometric analysis of mouse lymphocytes with Sell recombinant monoclonal antibody, clone MEL-14 (Cat # RAB03316).
Mouse lymphocytes were stained with an anti-CD44 control (3.0- panel A) or the rmeric version of RAB03316 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.Flow Cytometry
Flow cytometric analysis of mouse splenocytes with Sell recombinant monoclonal antibody, clone MEL-14 (Cat # RAB03316).
Mouse splenocytes were stained with unimmunized r antibody (black line) or the rmeric version of RAB03316 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer. -
Gene Info — Sell
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