Products

Western Blot

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining of human lung tissue with CD55 recombinant monoclonal antibody, clone LU30 (Cat # RAB03309).
Anti-CD55 (Complement decay-accelerating factor) staining of paraffin embedded human lung tissue using the rmeric version of RAB03309. Antigen retreival was acheived by microwaving in citrate buffer (pH6)- followed by blocking with protein block serum-free buffer (Dako- cat. #X0909). Primary antibody incubation with RAB03309 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-r HRP secondary antibody (Dako cat#K4009) for 20 mins followed by Ddiaminobenzidine)- and counter staining with haematoxylin. Strong staining of the epithelial surface of alveoli may be observed. Recommended concentration- 2-4 ug/ml.

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of U937 cells with CD55 recombinant monoclonal antibody, clone LU30 (Cat # RAB03309).
U937 cells were stained with unimmunized r antibody (black line) or the rmeric version of RAB03309 (blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using an anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Entrez GeneID

1604

Gene Name

CD55

Gene Alias

CR, CROM, DAF, TC

Gene Description

CD55 molecule, decay accelerating factor for complement (Cromer blood group)

Omim ID

125240

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq

Other Designations

CD55 antigen|decay accelerating factor for complement