SPN recombinant monoclonal antibody, clone 84-3C1
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More Files
- More Functions
Western Blot
Western blot anaysis anaysis of Lane 1: Daudi cell lysate, Lane 2: K562 cell lysate and Lane 3: human bone marrow tissue lysate with SPN recombinant monoclonal antibody, clone 84-3C1 (Cat # RAB03281).
Immunofluorescence
Immunofluorescent staining of K562 cells with SPN recombinant monoclonal antibody, clone 84-3C1 (Cat # RAB03281).
K562 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03281 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03281- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of K562 cells with SPN recombinant monoclonal antibody, clone 84-3C1 (Cat # RAB03281).
K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03281(blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SPN.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against stimulated human leukocytes.
Reactivity
Human
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot anaysis anaysis of Lane 1: Daudi cell lysate, Lane 2: K562 cell lysate and Lane 3: human bone marrow tissue lysate with SPN recombinant monoclonal antibody, clone 84-3C1 (Cat # RAB03281).Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of K562 cells with SPN recombinant monoclonal antibody, clone 84-3C1 (Cat # RAB03281).
K562 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03281 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03281- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of K562 cells with SPN recombinant monoclonal antibody, clone 84-3C1 (Cat # RAB03281).
K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03281(blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — SPN
Entrez GeneID
6693Gene Name
SPN
Gene Alias
CD43, GPL115, LSN
Gene Description
sialophorin
Omim ID
182160Gene Ontology
HyperlinkGene Summary
Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.[supplied by OMIM
Other Designations
leukosialin|sialophorin (gpL115, leukosialin, CD43)|sialophorin (leukosialin, CD43)
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Interactome
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Pathway
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Disease
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