Fcgr2b recombinant monoclonal antibody, clone AT130-5
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More Files
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Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Fcgr2b recombinant monoclonal antibody, clone AT130-5 (Cat # RAB03225).
Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of RAB03225 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Fcgr2b.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against CD32-rat CD4 domain 3/4 fustion protein.
Reactivity
Mouse
Form
Liquid
Isotype
IgG
Recommend Usage
Immunofluorescence
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Fcgr2b recombinant monoclonal antibody, clone AT130-5 (Cat # RAB03225).
Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of RAB03225 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody. -
Gene Info — Fcgr2b
Entrez GeneID
14130Gene Name
Fcgr2b
Gene Alias
AI528646, CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a, Fcr-2, Fcr-3, Ly-17, Ly-m20, LyM-1, Lym-1
Gene Description
Fc receptor, IgG, low affinity IIb
Gene Ontology
HyperlinkGene Summary
IgG
Other Designations
Fc gamma RIIB|Fc-gamma-RII|FcgammaRIIB|OTTMUSP00000024374|OTTMUSP00000024375
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