Fcgr2b recombinant monoclonal antibody, clone 2.4G2
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More Files
- More Functions
Blocking
Blocking of Fc-receptors with Fcgr2b recombinant monoclonal antibody, clone 2.4G2 (Cat # RAB03173).
Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate- left and middle panel) as well as bone marrow-derived macrophages (BMDMs- right panel) were stained with a murine anti-human antibody (fluorescently lAF647) human-specific anti-CD16) with or without pre-incubation with RAB03173. Pre-incubation of cells with RAB03173 reduced non-specific binding of the fluorescently lntibody to all cell types analysed and shows that RAB03173 acts as an Fc-blocking reagent.
Flow Cytometry
Flow cytometric analysis of murine macrophages with Fcgr2b recombinant monoclonal antibody, clone 2.4G2 (Cat # RAB03173)
Murine bone marrow-derived macrophages (BMDMs) were stained with RAB03173)(as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control- a CD16/32-positive population is apparent.
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Specification
Product Description
Rat recombinant monoclonal antibody raised against mouse Fcgr2b.
Antibody Species
Rat
Immunogen
Original antibody is raised against mouse BALB/c Macrophage J774 Cell Line.
Reactivity
Mouse
Form
Liquid
Isotype
IgG2b kappa
Recommend Usage
Blocking
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry
Immunofluorescence
Immunoprecipitation
Blocking
Blocking of Fc-receptors with Fcgr2b recombinant monoclonal antibody, clone 2.4G2 (Cat # RAB03173).
Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate- left and middle panel) as well as bone marrow-derived macrophages (BMDMs- right panel) were stained with a murine anti-human antibody (fluorescently lAF647) human-specific anti-CD16) with or without pre-incubation with RAB03173. Pre-incubation of cells with RAB03173 reduced non-specific binding of the fluorescently lntibody to all cell types analysed and shows that RAB03173 acts as an Fc-blocking reagent.Flow Cytometry
Flow cytometric analysis of murine macrophages with Fcgr2b recombinant monoclonal antibody, clone 2.4G2 (Cat # RAB03173)
Murine bone marrow-derived macrophages (BMDMs) were stained with RAB03173)(as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control- a CD16/32-positive population is apparent. -
Gene Info — Fcgr2b
Entrez GeneID
14130Gene Name
Fcgr2b
Gene Alias
AI528646, CD32, F630109E10Rik, Fc[g]RII, FcgRII, Fcgr2, Fcgr2a, Fcr-2, Fcr-3, Ly-17, Ly-m20, LyM-1, Lym-1
Gene Description
Fc receptor, IgG, low affinity IIb
Gene Ontology
HyperlinkGene Summary
IgG
Other Designations
Fc gamma RIIB|Fc-gamma-RII|FcgammaRIIB|OTTMUSP00000024374|OTTMUSP00000024375
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