L1CAM recombinant monoclonal antibody, clone L1-14.10
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More Files
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Western Blot
Western blot analysis of Lane 1: HeLa cells, Lane 2: Kelly cells, Lane 3: human brain cerebellum and Lane 4: cerebral cortex tissue lysates with L1CAM recombinant monoclonal antibody, clone L1-14.10 (Cat # RAB03174).
Immunofluorescence
Immunofluorescent staining of HeLa cells with L1CAM recombinant monoclonal antibody, clone L1-14.10 (Cat # RAB03174).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG version of RAB03174 at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03174- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Hela cells with L1CAM recombinant monoclonal antibody, clone L1-14.10 (Cat # RAB03174).
Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of RAB03174 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human L1CAM.
Antibody Species
Mouse
Immunogen
Original antibody is raised against recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
Reactivity
Human
Form
Liquid
Isotype
IgG1 kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Lane 1: HeLa cells, Lane 2: Kelly cells, Lane 3: human brain cerebellum and Lane 4: cerebral cortex tissue lysates with L1CAM recombinant monoclonal antibody, clone L1-14.10 (Cat # RAB03174).Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of HeLa cells with L1CAM recombinant monoclonal antibody, clone L1-14.10 (Cat # RAB03174).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG version of RAB03174 at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03174- DAPI- merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (.1) followed by Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of Hela cells with L1CAM recombinant monoclonal antibody, clone L1-14.10 (Cat # RAB03174).
Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody (.1; isotype control- black line) or the mouse IgG1 version of RAB03174 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — L1CAM
Entrez GeneID
3897Gene Name
L1CAM
Gene Alias
CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAML1, S10, SPG1
Gene Description
L1 cell adhesion molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq
Other Designations
OTTHUMP00000025992|antigen identified by monoclonal antibody R1|neural cell adhesion molecule L1
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Interactome
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Pathway
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Disease
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