VCAM1 recombinant monoclonal antibody, clone B-K9 (C106-K9)
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human VCAM1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against activated human umbilical vein endothelial cells.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Blocking
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of human peripheral blood leukocytes with VCAM1 recombinant monoclonal antibody, clone B-K9 (C106-K9) (Cat # RAB03147).
Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of RAB03147 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03147- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.Blocking
Flow Cytometry
Flow cytometric analysis of K562 cells with VCAM1 recombinant monoclonal antibody, clone B-K9 (C106-K9) (Cat # RAB03147).
Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03147 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — VCAM1
Entrez GeneID
7412Gene Name
VCAM1
Gene Alias
CD106, DKFZp779G2333, INCAM-100, MGC99561
Gene Description
vascular cell adhesion molecule 1
Omim ID
192225Gene Ontology
HyperlinkGene Summary
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
CD106 antigen|OTTHUMP00000012650|OTTHUMP00000012651
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