VCAM1 recombinant monoclonal antibody, clone R02-7H1
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human VCAM1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human VCAM1
Theoretical MW (kDa)
Calculated MW: 81 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1,000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of VCAM1 in 3T3 lysates using human VCAM1 recombinant monoclonal antibody, clone R02-7H1 (Cat # RAB01653).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with VCAM1 recombinant monoclonal antibody, clone R02-7H1 (Cat # RAB01653). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — VCAM1
Entrez GeneID
7412Protein Accession#
P19320Gene Name
VCAM1
Gene Alias
CD106, DKFZp779G2333, INCAM-100, MGC99561
Gene Description
vascular cell adhesion molecule 1
Omim ID
192225Gene Ontology
HyperlinkGene Summary
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
CD106 antigen|OTTHUMP00000012650|OTTHUMP00000012651
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