MARCKS recombinant monoclonal antibody, clone MARCKSS167170-C9
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More Files
- More Functions
Western Blot
Western blot analysis of NIH3T3 cell extract untreated or treated with TPA using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.1 ug/mL.
Flow Cytometry
Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or treated with staurosporine (red) or with UV+TPA (green) using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.01 ug/mL.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MARCKS.
Antibody Species
Rabbit
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser167/170 of human phospho MARCKS
Reactivity
Human
Form
Liquid
Purification
Protein A+G
Isotype
Rabbit IgG1k
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
1X PBS, 0.02% Sodium azide, 50% Glycerol, 0.1% BSA
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of NIH3T3 cell extract untreated or treated with TPA using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.1 ug/mL.Flow Cytometry
Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or treated with staurosporine (red) or with UV+TPA (green) using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.01 ug/mL. -
Gene Info — MARCKS
Entrez GeneID
4082Protein Accession#
P29966Gene Name
MARCKS
Gene Alias
80K-L, FLJ14368, FLJ90045, MACS, PKCSL, PRKCSL
Gene Description
myristoylated alanine-rich protein kinase C substrate
Omim ID
177061Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000017045|myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)|phosphomyristin
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Interactome
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Pathway
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