H3-4 recombinant monoclonal antibody, clone HisH3S28-D6
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More Files
- More Functions
Western Blot
Western blot analysis of Hela cell extract untreated or synchronized in metaphase by treatment with 0.4 ug/mL of nocodazole for 6 hr then metaphase cells were isolated by mitotic shake-off using Phospho-Histone H3 (Ser28) antibody at 1 ng/mL HisH3S28-D6.
Flow Cytometry
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 ug/mL of isotype control (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.1 ug/mL.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human H3-4.
Antibody Species
Rabbit
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human phospho Histone H3
Reactivity
Human
Form
Liquid
Purification
Protein A+G
Isotype
Rabbit IgG1k
Recommend Usage
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
1X PBS, 0.02% Sodium azide, 50% Glycerol, 0.1% BSA
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Hela cell extract untreated or synchronized in metaphase by treatment with 0.4 ug/mL of nocodazole for 6 hr then metaphase cells were isolated by mitotic shake-off using Phospho-Histone H3 (Ser28) antibody at 1 ng/mL HisH3S28-D6.Flow Cytometry
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 ug/mL of isotype control (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-Histone H3 (Ser28) antibody HisH3S28-D6 at 0.1 ug/mL. -
Gene Info — HIST3H3
Entrez GeneID
8290Protein Accession#
Q16695Gene Name
HIST3H3
Gene Alias
H3.4, H3/g, H3FT, H3t, MGC126886, MGC126888
Gene Description
histone cluster 3, H3
Omim ID
602820Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H3 histone family, member T|OTTHUMP00000037945|histone 3, H3
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Interactome
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Pathway
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