VTN recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The liver tissue lysate of Human Lane2:Human serum with VTN recombinant monoclonal antibody (Cat # RAB02759) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver tissue using VTN recombinant monoclonal antibody (Cat # RAB02759).Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of A549 cells using VTN recombinant monoclonal antibody (Cat # RAB02759)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against VTN.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant VTN.
Theoretical MW (kDa)
54, 75
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Vitronectin and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The liver tissue lysate of Human Lane2:Human serum with VTN recombinant monoclonal antibody (Cat # RAB02759) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver tissue using VTN recombinant monoclonal antibody (Cat # RAB02759).Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of A549 cells using VTN recombinant monoclonal antibody (Cat # RAB02759)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — VTN
Entrez GeneID
7448Protein Accession#
P04004Gene Name
VTN
Gene Alias
V75, VN, VNT
Gene Description
vitronectin
Omim ID
193190Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq
Other Designations
complement S-protein|epibolin|serum spreading factor|somatomedin B|vitronectin (serum spreading factor, somatomedin B, complement S-protein)
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Interactome
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Pathway
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Disease
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