GRM5 recombinant monoclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using GRM5 recombinant monoclonal antibody (Cat # RAB02757). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of SHG-44 cells cells using GRM5 recombinant monoclonal antibody (Cat # RAB02757)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against GRM5.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant GRM5.
Theoretical MW (kDa)
132
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of mGluR-5 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:200-1:500)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using GRM5 recombinant monoclonal antibody (Cat # RAB02757). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of SHG-44 cells cells using GRM5 recombinant monoclonal antibody (Cat # RAB02757)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — GRM5
Entrez GeneID
2915Protein Accession#
P41594Gene Name
GRM5
Gene Alias
GPRC1E, MGLUR5, mGlu5
Gene Description
glutamate receptor, metabotropic 5
Omim ID
604102Gene Ontology
HyperlinkGene Summary
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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