ACTA2 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against ACTA2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant ACTA2.
Theoretical MW (kDa)
42
Reactivity
Human, Mouse, Rat, Zebra fish
Specificity
This antibody detects endogenous levels of alpha-SMA and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:A549 whole cell lysate Lane2:Hela whole cell lysate Lane3:NIH/3T3 whole cell lysate Lane4:The heart tissue lysate of Mouse with ACTA2 recombinant monoclonal antibody (Cat # RAB02738) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung tissue using ACTA2 recombinant monoclonal antibody (Cat # RAB02738). Counter stained with hematoxylin.Flow Cytometry
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Gene Info — ACTA2
Entrez GeneID
59Protein Accession#
P62736Gene Name
ACTA2
Gene Alias
AAT6, ACTSA
Gene Description
actin, alpha 2, smooth muscle, aorta
Omim ID
102620Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000020042|alpha 2 actin|alpha-cardiac actin|growth-inhibiting gene 46
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