PIK3R3 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against PIK3R3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant PIK3R3.
Theoretical MW (kDa)
55
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of PI3K p55gamma and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:200-1:500)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:MCF7 whole cell lysate Lane2:Jurkat whole cell lysate Lane3:The testis tissue lysate of Mouse with PIK3R3 recombinant monoclonal antibody (Cat # RAB02696) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using PIK3R3 recombinant monoclonal antibody (Cat # RAB02696). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of PC-3M cells using PIK3R3 recombinant monoclonal antibody (Cat # RAB02696)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — PIK3R3
Entrez GeneID
8503Protein Accession#
Q92569Gene Name
PIK3R3
Gene Alias
DKFZp686P05226, FLJ41892, p55, p55-GAMMA
Gene Description
phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Omim ID
606076Gene Ontology
HyperlinkGene Summary
O
Other Designations
100% homology to SWISS-PROT Q92569|OTTHUMP00000009783|OTTHUMP00000009786|phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma)|phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma)|phosphoinositide-3-kinase, regulatory subun
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Interactome
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Pathway
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Disease
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