DUSP6 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:The pancreas tissue lysate of Mouse with DUSP6 recombinant monoclonal antibody (Cat # RAB02686) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using DUSP6 recombinant monoclonal antibody (Cat # RAB02686). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using DUSP6 recombinant monoclonal antibody (Cat # RAB02686)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against DUSP6.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant DUSP6.
Theoretical MW (kDa)
42
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of DUSP6 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:200-1:500)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:The pancreas tissue lysate of Mouse with DUSP6 recombinant monoclonal antibody (Cat # RAB02686) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using DUSP6 recombinant monoclonal antibody (Cat # RAB02686). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using DUSP6 recombinant monoclonal antibody (Cat # RAB02686)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — DUSP6
Entrez GeneID
1848Protein Accession#
Q16828Gene Name
DUSP6
Gene Alias
MKP3, PYST1
Gene Description
dual specificity phosphatase 6
Omim ID
602748Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
MAP kinase phosphatase 3|serine/threonine specific protein phosphatase
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Interactome
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