KRT20 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Lovo whole cell lysate Lane2:HCT116 whole cell lysate Lane3:CRC whole cell lysate with KRT20 recombinant monoclonal antibody (Cat # RAB02675) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using KRT20 recombinant monoclonal antibody (Cat # RAB02675). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of CRC cells using KRT20 recombinant monoclonal antibody (Cat # RAB02675)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against KRT20.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant KRT20.
Theoretical MW (kDa)
48
Reactivity
Human, Rat
Specificity
This antibody detects endogenous levels of Cytokeratin 20 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:10-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Lovo whole cell lysate Lane2:HCT116 whole cell lysate Lane3:CRC whole cell lysate with KRT20 recombinant monoclonal antibody (Cat # RAB02675) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using KRT20 recombinant monoclonal antibody (Cat # RAB02675). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of CRC cells using KRT20 recombinant monoclonal antibody (Cat # RAB02675)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — KRT20
Entrez GeneID
54474Protein Accession#
P35900Gene Name
KRT20
Gene Alias
CD20, CK20, K20, KRT21, MGC35423
Gene Description
keratin 20
Omim ID
608218Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
cytokeratin 20|keratin, type I cytoskeletal 20
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Interactome
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Disease
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