RPS3 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:PC12 whole cell lysate with RPS3 recombinant monoclonal antibody (Cat # RAB02668) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using RPS3 recombinant monoclonal antibody (Cat # RAB02668). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of N2A cells using RPS3 recombinant monoclonal antibody (Cat # RAB02668)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against RPS3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant RPS3.
Theoretical MW (kDa)
26
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of RPS3 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:PC12 whole cell lysate with RPS3 recombinant monoclonal antibody (Cat # RAB02668) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human kidney tissue using RPS3 recombinant monoclonal antibody (Cat # RAB02668). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of N2A cells using RPS3 recombinant monoclonal antibody (Cat # RAB02668)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — RPS3
Entrez GeneID
6188Protein Accession#
P23396Gene Name
RPS3
Gene Alias
FLJ26283, FLJ27450, MGC87870
Gene Description
ribosomal protein S3
Omim ID
600454Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
40S ribosomal protein S3|IMR-90 ribosomal protein S3
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Interactome
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Pathway
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