CREB1 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against CREB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant CREB1.
Theoretical MW (kDa)
38
Reactivity
Human, Mouse, Rat, Zebra fish
Specificity
This antibody detects endogenous levels of CREB and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:10-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:HepG2 whole cell lysate Lane3:Jurkat whole cell lysate Lane4:NIH/3T3 whole cell lysate with CREB1 recombinant monoclonal antibody (Cat # RAB02660) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CREB1 recombinant monoclonal antibody (Cat # RAB02660). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using CREB1 recombinant monoclonal antibody (Cat # RAB02660)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — CREB1
Entrez GeneID
1385Protein Accession#
P16220Gene Name
CREB1
Gene Alias
CREB, MGC9284
Gene Description
cAMP responsive element binding protein 1
Omim ID
123810Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
active transcription factor CREB|cAMP-response element-binding protein-1|transactivator protein
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Interactome
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