CREB1 (Phospho S133) recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of C6 UV treated (A) whole cell lysates with CREB (Phospho S133) recombinant monoclonal antibody (Cat # RAB02486).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using CREB (Phospho S133) recombinant monoclonal antibody (Cat # RAB02486). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.79). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CREB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB.
Theoretical MW (kDa)
43
Reactivity
Human, Rat
Specificity
Recognizes endogenous levels of CREB (pS133) protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:10-1:50)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of C6 UV treated (A) whole cell lysates with CREB (Phospho S133) recombinant monoclonal antibody (Cat # RAB02486).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human lung cancer formalin fixed paraffin embedded tissue section using CREB (Phospho S133) recombinant monoclonal antibody (Cat # RAB02486). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.79). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — CREB1
Entrez GeneID
1385Protein Accession#
P16220Gene Name
CREB1
Gene Alias
CREB, MGC9284
Gene Description
cAMP responsive element binding protein 1
Omim ID
123810Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
active transcription factor CREB|cAMP-response element-binding protein-1|transactivator protein
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