ITGA6 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis with ITGA6 recombinant monoclonal antibody (Cat # RAB02638) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded human tonsil using ITGA6 recombinant monoclonal antibody (Cat # RAB02638). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ITGA6.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant human ITGA6.
Theoretical MW (kDa)
120
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of Integrin alpha 6 protein.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.01M TBS, pH7.4 (1% BSA, 0.03% Proclin300 and 50% Glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles. -
Applications
Western Blot (Tissue lysate)
Western blot analysis with ITGA6 recombinant monoclonal antibody (Cat # RAB02638) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded human tonsil using ITGA6 recombinant monoclonal antibody (Cat # RAB02638). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C. -
Gene Info — ITGA6
Entrez GeneID
3655Protein Accession#
P23229Gene Name
ITGA6
Gene Alias
CD49f, DKFZp686J01244, FLJ18737, ITGA6B, VLA-6
Gene Description
integrin, alpha 6
Gene Ontology
HyperlinkGene Summary
The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
integrin alpha chain, alpha 6|integrin alpha6B
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Interactome
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Pathway
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Disease
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