CRTC2 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A549 (A), HL60 (B), U251 (C) whole cell lysates with CRTC2 recombinant monoclonal antibody (Cat # RAB02615).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using CRTC2 recombinant monoclonal antibody (Cat # RAB02615). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.27). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CRTC2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human CRTC2.
Theoretical MW (kDa)
80
Reactivity
Human
Specificity
Recognizes endogenous levels of TORC2 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A549 (A), HL60 (B), U251 (C) whole cell lysates with CRTC2 recombinant monoclonal antibody (Cat # RAB02615).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human breast carcinoma formalin fixed paraffin embedded tissue section using CRTC2 recombinant monoclonal antibody (Cat # RAB02615). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.27). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — CRTC2
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Interactome
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Disease
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