RPS20 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RPS20.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human RPS20.
Theoretical MW (kDa)
15
Reactivity
Hamster, Human, Mouse, Rat
Specificity
Recognizes endogenous levels of RPS20 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NIH3T3 (A), Raw264.7 (B), PC12 (C), C6 (D), Hela (E) whole cell lysates with RPS20 recombinant monoclonal antibody (Cat # RAB02607).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human tonsil formalin fixed paraffin embedded tissue section using RPS20 recombinant monoclonal antibody (Cat # RAB02607). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.131). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. -
Gene Info — RPS20
Entrez GeneID
6224Protein Accession#
P60866Gene Name
RPS20
Gene Alias
FLJ27451, MGC102930
Gene Description
ribosomal protein S20
Omim ID
603682Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
40S ribosomal protein S20
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